Welcome to the wide wonderful world of telomere length quantification through image analysis!
Telometer was created by Johns Hopkins University. It runs as a plugin for the ImageJ program.
The source code for Telometer is available for download on this site. ImageJ was developed by the NIH. From here you can download the program Telometer for use in quantifying telomere (or other FISH) signals from uncompressed 8, 16 or 24 bit gray scale TIFF images. In order to run the program you will need to have installed the free image analysis program ImageJ. You will also need a matched pair of TIFF images, one with your FISH signals, one with images of the cell nuclei (we use the DNA-binding dye DAPI for this purpose). Included here you will find: A Zip file containing the image analysis tool “Telometer,” a plugin for the open source Java-based image analysis program ImageJ. The MS WORD document “Install notes for Telometer plugin for ImageJ” that describes the installation and use of the Telometer plugin written for the image analysis program ImageJ (as well as locating and installing the free open source program ImageJ). A folder containing two monochrome test images (16 bit TIFF format, greyscale) for your use in trying out Telometer. Also included in the folder is a text file describing the images as well as a third triple color overlay image (8 bit RGB TIFF) showing the area from which the monochrome images were taken. While this plugin was originally designed for extracting quantitative information from FISH images of telomeric DNA in interphase nuclei, it has also been applied to FISH images using a pan-centromeric probe, and should be generally applicable to FISH images where the amount of target is of interest and is related either to the signal intensity or the number of signals.